Open AccessMolecular Epidemiology of Epstein-Barr Virus in Women Breast Cancer in Congo Brazzaville
Author(s) -
Dimitry Moudiongui Mboungou Malanda,
Anicet Luc Magloire Boumba,
Fabien Mouamba
Publication year - 2021
Publication title -
international journal of health sciences and research
Language(s) - English
Resource type - Journals
ISSN - 2249-9571
DOI - 10.52403/ijhsr.20211124
Subject(s) - breast cancer , medicine , cancer , polymerase chain reaction , immunohistochemistry , virus , pathology , oncology , cancer research , virology , biology , gene , genetics
the Epstein Barr virus is one of the very first oncogenic viruses to be identified as responsible for human malignancies. Its role as an etiological agent of breast cancer remains controversial, however, despite the growing molecular evidence. The aim of this study was detected the presence of EBV DNA in patients with breast cancer in the Republic of Congo.Methods: The study was conducted on 90 samples of formalin fixed and paraffin-embedded tissue blocks (FFPE) from breast cancer tissue. The immunohistochemistry technique was used to test for the expression of the LMP1 antibody and DNA was extracted from all blocks of formalin-fixed and paraffin-embedded breast cancer tissue (FFPE) to detect presence of EBV 1 DNA by real-time polymerase chain reaction (PCR).Results: EBV was detected in 12.33% (12/90) of formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue blocks. All formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue blocks with positive EBV DNA were high tumor grades (II and III). Overall EBV infection with clinicopathological features of breast cancer cases showed no significant difference (P>0.05). However, a statistically significant difference was observed between EBV infection and histological types (P=0.04).Conclusion: Our results provide evidence for the presence of EBV DNA in female breast cancer in Congo Brazzaville. However, this evidence is substantial but inconclusive for the involvement of viruses in the development of breast cancer. Therefore, future investigations will be needed to elucidate the exact role of EBV in breast cancer in women in the Republic of Congo.Key words: EBV, breast cancer, women, Congo Brazzaville.