Open AccessMEMBRANE TECHNOLOGY IN THE EXTRACTION OF INDOLE ALKALOIDS
Author(s) -
V. Yu. Vachnadze,
NINA VACHNADZE VACHNADZE,
A Bakuridze,
RAUL GOCIRIDZE,
Malkhaz Jokhadze,
VAXTANG MSHVILDADZE,
LAMZIRA EBRALIDZE,
DALI BERASHVILI
Publication year - 2022
Publication title -
ek'sperimentuli da klinikuri medic'ina
Language(s) - English
Resource type - Journals
ISSN - 1512-0392
DOI - 10.52340/jecm.2022.723
Subject(s) - chemistry , fractionation , ultrafiltration (renal) , membrane , chromatography , fraction (chemistry) , monomer , extraction (chemistry) , indole test , apocynaceae , stereochemistry , organic chemistry , biochemistry , botany , biology , polymer
By using ultrafiltration membrane with a certain porosity, it is possible to achieve selective fractionation of dimeric and monomeric alkaloids at the molecular weight level. With porosity of the membrane 100-150A° and filtration of alcohol extracts of Vinca rosea L. obtained from the aerial part of the plant, selective fractionation was achieved within the molecular masses from M+ 168 to M+ 426. In the fraction of minor alkaloids, a norharman (M+ 168), dihydrositsirikin (M+ 356) and vindorosine (M+ 426) were identified by the method of LC-MS/MS and GC/MS. The fraction of the minor alkaloids showed strong cytotoxic activity against the DLD-1 and WS-1 cell lines (Resazurin test), and according to the Hoechst test, to the cells of all A-549, DLD-1 and WS-1 lines.