Open AccessCausative Pathogen of Fire Blight of Fruit Trees in Georgia
Author(s) -
Nanuli Amashukeli,
Dali Gaganidze,
Mariam Aznarashvili,
Shorena Kharadze,
Neli Sturua,
Tinatin Sadunishvili
Publication year - 2021
Publication title -
šromebi
Language(s) - English
Resource type - Journals
ISSN - 2233-3401
DOI - 10.52340/idw.2021.488
Subject(s) - fire blight , blight , pathogen , erwinia , fungal pathogen , biology , horticulture , rosaceae , botany , veterinary medicine , microbiology and biotechnology , medicine , gene , genetics
Fire blight is a devastating disease of fruit trees that first appeared in Georgia in 2016. Above 40 samples – fruit trees twigs, buds, flowers and fruitlets, suspected on the fire blight disease were collected in the Mtskheta-Mtianeti and Shida Kartli Regions, eastern Georgia in Summer of 2020. Based on visual observation and immunological test (Ea AgriStrip), 20 plant samples were selected for further study. PCR analysis was performed to detect pathogen in these samples with primers pair A: 5′-CGG TTT TTA ACG CTG GG-3′ and B: 5′-GGG CAA ATA CTC GGA TT-3 ′ and 2XPCR BIOTAG Mix using PCR BIOSYSTEMS. The pathogen Erwinia amylovora was detected in 6 samples: N10 and N11 apple samples (Ksovrisi);