Open AccessANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC EVALUATION OF VARIOUS EXTRACTS OF IFLOGA SPICATA (FORSSK.) SCH.BIP
Author(s) -
Yahya Khan,
S. Jan,
Farhat Ullah,
Aamir Khan Awan,
Norhayati Muhammad,
Syed A. A. Rizvi
Publication year - 2021
Publication title -
latin american applied research
Language(s) - English
Resource type - Journals
eISSN - 1851-8796
pISSN - 0327-0793
DOI - 10.52292/j.laar.2021.733
Subject(s) - dpph , ethyl acetate , chloroform , chemistry , antioxidant , antimicrobial , hydrogen peroxide , phytochemical , tbars , mentha spicata , traditional medicine , food science , biochemistry , organic chemistry , essential oil , lipid peroxidation , medicine
The current study reveals the pharmacological and phytochemical potential of different solvent fractions of Ifloga spicata. Antimicrobial and free radical scavenging activities, DPPH (1, 1-diphenyl-2-picrylhydrazyl), hydrogen peroxide scavenging assay, and cytotoxic effects of plant samples on lymphocytes stressed with hydrogen were performed. Ifloga spicata ethyl acetate fraction markedly showed higher antibacterial potential against P. aeruginosa (ATCC 27853) and E. Coli (ATCC 25922) bacteria. While higher antifungal potential was shown by the chloroform fraction against all three different a strain A. fumigates (ATCC 204305), A. flavus (ATCC 9643) and A. niger (ATCC 16404) as compared to others fractions of sample. DPPH maximum antioxidant potential was noted for methanolic extract followed by ethyl acetate and chloroform. Similarly, hydrogen peroxide effect was significantly decreased by ethyl acetate fraction. In blood lymphocytes the raise in ROS and TBARS level due to stress given by H2O2 was also recovered by different extracts of I. spicata. The decrease level of CAT, SOD and POD was also restored