Open AccessThe Investigation of Streptococcus Agalactiae Colonization in Last Trimester Pregnants by Using Standard Culture and Molecular Methods
Author(s) -
N Kuru,
Oğuzhan Kuru,
Abdullah Tüten,
Nevriye Gönüllü
Publication year - 2021
Publication title -
i̇zmir tepecik eğitim hastanesi dergisi
Language(s) - English
Resource type - Journals
eISSN - 1305-7146
pISSN - 1305-7073
DOI - 10.5222/terh.2021.99266
Subject(s) - gold standard (test) , streptococcus agalactiae , colonization , medicine , obstetrics , group b , streptococcus , obstetrics and gynaecology , microbiological culture , gynecology , pregnancy , biology , microbiology and biotechnology , bacteria , genetics
Objective: We aimed to detect and compare group B streptococcus (GBS) colonization in pregnant women at third trimester using real-time polymerase chain reaction (real-time PCR) and culture methods. Methods: Vaginal swab specimens were taken for screening of GBS from 100 women between 35-37 weeks of gestation who were attending to antenatal outpatient unit of Obstetrics and Gynecology Department of Cerrahpasa Medical Faculty from May 2014 to September 2014. Results: Rates of GBS colonization was %5 and %7 by culture and real-time PCR methods, respectively. Using culture as the gold standard; sensitivity and specificity for real-time PCR were 100% and 97.9%, respectively. Any significant difference was not detected between GBS colonization with age groups, education levels, number of previous pregnancies, smoking habits, history of antibiotic use, and contraceptive method. Conclusion: Real-time PCR technique has proven to be as sensitive as the culture method. Also, real-time PCR may provide a rapid diagnostic tool for GBS detection potentially allowing a more effective intrapartum antibiotic prophylaxis and lower infant morbidity and mortality. However, the inability to use PCR test in every laboratory and its high cost creates a handicap.