Open AccessInfection Related Immunoglobulin-M Antibodies Cross Reactive with Human Leucocyte Antigens
Author(s) -
Derya Güleç,
Tülay Kılıçaslan Ayna,
Mustafa Soyöz,
Nisel Yılmaz,
Cem Şirin,
İbrahim Pirim
Publication year - 2021
Publication title -
i̇zmir tepecik eğitim hastanesi dergisi
Language(s) - English
Resource type - Journals
eISSN - 1305-7146
pISSN - 1305-7073
DOI - 10.5222/terh.2021.90692
Subject(s) - immunology , antigen , antibody , medicine , virology , rubella , complement fixation test , human leukocyte antigen , cytomegalovirus , virus , serology , vaccination , herpesviridae , measles , viral disease
Objective: The first required feature for the patients, who will undergo allotransplantation, is HLA compatibility between donor and the recipient. In this study, it was aimed to indicate whether IgM antibodies that are produced during acute infection phase affect crossmatch tests or not, in addition to hitches that can occur in HLA incompatible transplants. Methods: Eighty-two patients with acute infection due to only one infectious agent and high serum IgM antibody levels were involved in this study. The patients had no alloimmunization (blood transfusion, pregnancy, and/or previous transplants). Fifty-five healthy individuals were used as HLA antigen source in order to evaluate Ab-Ag lymphocytotoxicity reactions. Results: The infectious agent distribution in the sera samples were as 25.6% Anti-Epstein-Barr virus capsid antigen (EBV VCA), 14.6% Anti-Cytomegalovirus (CMV), 17.1% Anti-Toxoplasma, 11% Anti-Hepatitis A Virus (HAV), 7.3% Anti-Rubella, 7.3% Anti-Varicella zoster virus (VZV), 4.9% Anti-Brucella, 3.7% Anti-Mumps, 2.97% Anti-Parvovirus B19, 1.9% Anti-Herpes simplex virus (HSV)-1, and 3.7% Anti-HBc. IgM antibody and HLA antigen reactions were analyzed by lymphocytotoxicity method in terasaki plates using peripheral blood lymphocytes of 55 healthy individuals. Conclusion: In lymphocytotoxicity test, the distribution of reactions that IgM antibodies gave to HLA antigens without class I-II differentiation according to infections were identified as 50% Brucella, 50% VZV, 44.4% HAV, 41.7% CMV, 42.9% EBV, 35.7% Toxoplasma, and 33.3% Rubella.