Open AccessAntioxidant activities of snakehead (Channa striata) fish skin: peptides hydrolysis using protease tp2 isolate from swamp plant silage
Author(s) -
Ace Baehaki,
Indah Widiastuti,
Citra H. Nainggolan,
Nuni Gofar
Publication year - 2020
Publication title -
potravinárstvo
Language(s) - English
Resource type - Journals
eISSN - 1338-0230
pISSN - 1337-0960
DOI - 10.5219/1264
Subject(s) - snakehead , hydrolysis , hydrolysate , antioxidant , swamp , protease , enzymatic hydrolysis , dpph , silage , food science , chemistry , biology , biochemistry , enzyme , fish <actinopterygii> , fishery , ecology
The purpose of this research was to study the antioxidants activities of peptides from skin fish of snakehead (Channa striata), using hydrolysis of protease TP2 isolate from swamp plant silage. This research 5 treatments hydrolysis time (0, 30, 60, 90, 120 min, respectively), with two replicates, which included several stages of preparation and pre-treatment of the snakehead fish skin production of protease enzymes which were isolated from swamp water, preparation of protein hydrolysates, measurement of hydrolysis degrees, analysis of peptides content and analysis of the antioxidant activity. Results showed that the treatment had given a significant effect on the 5% level of the degree of hydrolysis production (13.98% – 27.08%), with peptides content of 2.73% – 3.78% and antioxidant activity (10.75% – 20.7%). The results of the degree of hydrolysis indicate that the longer the hydrolysis time, the percent degree of hydrolysis will increase. Peptide content and antioxidant activity were increased with increasing hydrolysis time.