Open AccessFirst occurrence of Xanthomonas campestris pv. raphani on wallflower ( Erysimum cheiri ) in the United Kingdom
Author(s) -
Stead D.E.,
Parkinson N.,
Aspin A.,
Heeney J.,
Crump S.,
Vicente J.G.
Publication year - 2016
Publication title -
new disease reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.184
H-Index - 4
ISSN - 2044-0588
DOI - 10.5197/j.2044-0588.2016.034.014
Subject(s) - xanthomonas campestris , wilting , biology , botany , microbiology and biotechnology , xanthomonas campestris pv. campestris , bacteria , genetics
In a study of diseases caused by Xanthomonas campestris on UK nursery-grown wallflowers (Erysimum cheiri), one isolate (P764) obtained in 1990 from Sussex differed from the others. The affected plants (cv. Bedder Mixed), showed distorted growth with chlorotic and necrotic spots, but no sectored V-shaped wilting, the most common symptom associated with xanthomonads in wallflowers. Other X. campestris isolates (including P763), were obtained from wallflowers with typical wilting. Infected plant material was comminuted in sterile 0.1% peptone solution and loopfuls streaked onto yeast dextrose chalk agar and nutrient dextrose agar (Lelliott & Stead, 1987). Plates were incubated for up to 72 hours at 28°C. Xanthomonas-like colonies were purified by re-streaking and isolates were maintained at -80°C (Protect System, UK).