Open AccessFirst report of Tomato leaf curl Sinaloa virus infecting tomato crops in Panama
Author(s) -
HerreraVásquez J.A.,
Ortega D.,
Romero A.B.,
Davino S.,
Mejía L.C.,
Panno S.,
Davino M.
Publication year - 2015
Publication title -
new disease reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.184
H-Index - 4
ISSN - 2044-0588
DOI - 10.5197/j.2044-0588.2015.031.030
Subject(s) - biology , begomovirus , panama , solanum , leaf curl , plant virus , tomato yellow leaf curl virus , virus , polymerase chain reaction , genbank , virology , botany , horticulture , gene , genetics , ecology
In April 2011 and September 2012, virus-like symptoms were observed in open field- and greenhouse-grown tomato crops (Solanum lycopersicum) in Chiriquí, the westernmost province of Panama. Samples from symptom-bearing plants (127 in all) were collected and tested for the presence of begomoviruses by polymerase chain reaction (PCR) assays with sets of degenerated primers designed to amplify parts of the DNA-A and DNA-B components (Rojas et al., 1993; Table 1). Products of the expected sizes, obtained with both DNA-A- and DNA-B-specific primers for 49 samples, suggested infection with New World bipartite begomoviruses. This corresponds to an incidence of 26% (8 plants) in open field, and 43% (41 plants) in greenhouse crops. Primers specific for ten tomato-infecting begomoviruses found in Central America (Engel et al., 1998; Nakhla et al., 2005; Table 1) were used to typify the PCR-positive samples