Open AccessEffects of homeopathic medicines on macrophage - E. cuniculi interaction in vitro
Author(s) -
Leoni Villano Bonamin,
Mirian Yaeko Do Nagai,
Luciane Costa Dalboni,
Thayná Neves Cardoso,
Michelle S Correia,
Renata Rossetini Palombo,
Sandra Ag Pinto,
Cidéli de Paula Coelho,
Maria Anete Lallo
Publication year - 2021
Publication title -
international journal of high dilution research
Language(s) - English
Resource type - Journals
ISSN - 1982-6206
DOI - 10.51910/ijhdr.v17i1.890
Subject(s) - encephalitozoon cuniculi , acridine orange , in vitro , microbiology and biotechnology , biology , incubation , phagocytosis , intracellular , chemistry , biochemistry , apoptosis , microsporidia , spore
Encephalitozoon cuniculi (E. cuniculi) is a fungus that behaves as an intracellular parasite infecting different types of cells. In rabbits and immunosuppressed animals of other species, including humans, it parasites neural tissues causing a highly characteristic neurological syndrome, for which repertory analysis indicates Phosphorus. Successful treatment of 7 naturally infected rabbits with Phosphorus confirmed that this medicine probably is the remedy of the ‘epidemic genius’ of microsporidiosis. In the present study, an in vitro model was used to evidence the intracellular relationship between macrophages and E. cuniculi after treatment of co-cultures with different potencies of Phosphorus. RAW 254.7 macrophages were co-cultivated with E. cuniculi and treated with Phosphorus 6cH, 30cH and 200cH. Controls were untreated co-cultures and cultures treated with vehicle (0.06% final alcohol concentration). Phagocytosis and lysosome activity were after after 1 and 24 hours of incubation by means of the calcofluor and acridine orange staining methods, followed by automatic image analysis (Metamorph®). Cytokine production was assessed with the MAGPIX®-Luminex system. The vehicle increased IL-6, MCP-1 and MIP1 production (p