Open AccessStudy of the action mechanisms of Arnica montana effects on macrophages
Author(s) -
Marta Marzotto,
Clara Bonafini,
Debora Olioso,
Paolo Bellavite
Publication year - 2021
Publication title -
international journal of high dilution research
Language(s) - English
Resource type - Journals
ISSN - 1982-6206
DOI - 10.51910/ijhdr.v15i4.861
Subject(s) - thp1 cell line , gene , phagocytosis , biology , microbiology and biotechnology , traditional medicine , cell culture , chemistry , immunology , genetics , medicine
Objective: To test the effect of Arnica montana (Arnica m.) on human macrophages.
Method: The human monocytic leukaemia cell line THP-1 was cultured and differentiated in mature macrophages with PMA and other differentiating agents. Macrophages were exposed to Arnica m. homeopathic dilutions (2c, 3c, 5c, 9c and 15c) or Control solvent. Total RNA was isolated and sequenced to perform quantitative evaluation.
Results: Screening sorted out with a list of 20 genes that were significantly changed by Arnica m. 2c treatment: 7 up-regulated and 13 down-regulated. Most notably, a clearly up-regulated function concerned the proteinaceous extracellular matrix (ECM), including genes HSPG2, FBN2, FN1 (p