Open AccessRaman spectroscopy shows difference in drugs at ultra high dilution prepared with stepwise mechanical agitation
Author(s) -
Tandra Sarkar,
Atheni Konar,
Nirmal Chandra Sukul,
Dipanwita Majumdar,
Achintya Singha,
Anirban Sukul
Publication year - 2021
Publication title -
international journal of high dilution research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.129
H-Index - 9
ISSN - 1982-6206
DOI - 10.51910/ijhdr.v15i1.811
Subject(s) - raman spectroscopy , dilution , chemistry , hydrogen bond , serial dilution , analytical chemistry (journal) , spectral line , spectroscopy , molecule , chromatography , organic chemistry , thermodynamics , astronomy , quantum mechanics , optics , medicine , physics , alternative medicine , pathology
Objective
The present study aims at deciphering the nature of the water structure in two drugs at ultra high dilution (HD) by Laser Raman Spectroscopy.
Method
Two drugs like Sulphur and Natrum mur and their three high dilutions 30cH, 200cH and 1000cH were selected for the study. The 30cH means dilution 1060 with mechanical agitation in 30 steps.Raman spectra of the drugs and their medium (90%ethanol)were obtained in the wave number region of 2600-3800 cm-1. The intensity ratio at vibration frequencies between 3220 and 3420 (R1) and that between 3620 and 3420 (R2) were calculated for each HD as well as the control.
Results
Raman spectra show differences in intensities in different HD’s and their control in the stretching vibrations of CH and OH groups. The three HD’s of each drug show inverse relationship with respect to the R1 values. However, for R2 the relationship of HD’s for each drug is positive.
Conclusion
R1 provides information about the relative number of OH groups with strong and weak hydrogen bonds. R2 suggests the relative number of OH groups with broken and weak hydrogen bonds. Judged from R1 values the lower is the rank of HD, the stronger is the H-bond of the OH groups. In the light of R2 values the higher is the HD rank the more abundant is the free OH groups. So, hydrogen bond strength and free OH groups together make an effective HD rank relating to Sulphur and Natrum mur.