Open AccessClinical and parasitological assessment in mice treated with highly diluted Atropa belladonna
Author(s) -
Patrícia Flora Sandri,
Angela Rigo Portocarrero,
Larissa Ciupa,
Franciele Karina da Veiga,
Gislaine Falkowski Sanchez Falkowski,
Mateus Joacir Benvenutti,
Willian Nascimento de Souza Rodrigues,
Denise Lessa Aleixo,
Silvana Marques de AraÃojo
Publication year - 2021
Publication title -
international journal of high dilution research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.129
H-Index - 9
ISSN - 1982-6206
DOI - 10.51910/ijhdr.v13i47.734
Subject(s) - parasitemia , atropa belladonna , medicine , tincture (heraldry) , traditional medicine , serial dilution , biology , horticulture , malaria , immunology , plasmodium falciparum , alternative medicine , pathology
The infection by Trypanosoma cruzi is a public health problem and there is no effective treatment currently. Immunomodulatory effects of Atropa belladonna may offer benefits1 Objective: To evaluate the effect of A. belladonna in murine infection by T. cruzi. Methodology: The experiment was blind, controlled and randomized by draw. Eighty five Swiss male mice, at 8 weeks of age, were infected with 1400 blood trypomastigotes of T. cruzi Y strain (via IP) and divided into the following groups: without treatment (CI), treated with the mother tincture of A. belladonna (GTM-HN Cristiano), treated with A. belladonna 5cH (G5cH), treated with A. belladonna 6cH (G6cH), treated with A. belladonna 30cH (G30cH). Cereal alcohol 70 ° GL was used for dilutions as well as water in final preparations (Sigma-SP-Brazil). Oral treatment, diluted with water (1mL/100mL water), offered ad libitum 48 hours before infection, available during 16h. After infection, treatment of 56/56h for 16h, until the 9th day of infection2. Parasitological parameters: Curve of parasitemia, total parasitemia (PT), Maximum Peak of Parasites (PMP), Pre-Patent Period (PPP), Patent Period (PP), and Survival. Clinical parameters: water, food, excreta, weight and temperature. Results: G6cH and G30cH groups displayed better survival rates (1.54 and 1.42 times versus IC), and higher curve of parasitemia - G6cH (p = 0.00), G30cH (p = 0.02) – when compared to CI. PMP was lower in GTM (P = 0.01) and G5cH (p = 0.04) groups; PT was lower in GTM (p = 0.01), G5cH (p = 0.05) and G6cH (p = 0.05) groups when compared to CI. There was no difference in PPP and PP parameters in all groups, with a tendency of a higher PPP in G5cH and G6cH groups and lower PPP in G30cHgroup.The mice weight was higher in GTM (