Open AccessCausticum hahnemanni, Conium maculatum and Lycopodium clavatum highly diluted medications decreases parasitemia in mice infected by Trypanosoma cruzi
Author(s) -
Gislaine Janaina Sanchez Falkow Temporini,
Claudivan Sanches Lopes,
Camila Fernada Brustolin,
Paula Fernanda Massini,
Érika Cristina Ferreira,
Franciele Karina da Veiga,
Denise Lessa Aleixo,
Nelson Roberto Pala,
Terezinha Fátima Barion,
Luiz Gilson Esper,
Silvana Márques de Araújo
Publication year - 2021
Publication title -
international journal of high dilution research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.129
H-Index - 9
ISSN - 1982-6206
DOI - 10.51910/ijhdr.v11i40.591
Subject(s) - parasitemia , benznidazole , trypanosoma cruzi , medicine , inoculation , veterinary medicine , chagas disease , traditional medicine , biology , zoology , malaria , immunology , parasite hosting , plasmodium falciparum , world wide web , computer science
Benznidazole is the only medicine available in Brazil for Chagas’ disease treatment, however it presents low efficacy in the chronic phase and several adverse effects [1-3].
Aim: Evaluate the effect of Causticum hahnemanni, Conium maculatum and Lycopodium clavatum [4-6] administered to mice infected with T. cruzi.
Method: In blind randomized controlled trial 42 male Swiss mice, 8 weeks of age, have been grouped: GCaus –treated with C. hahnemanni 13cH (n=10), GCon –treated with C. maculatum 13 cH (n=11), GLy –treated with L. clavatum13cH (n=10) and CG – control group (n=11) treated with 7% hydro alcoholic solution 13cH. The animals were infected intraperitoneally with 1.400 blood trypomastigotes of T. cruzi - Y strain. Medications were been prepared according to Brazilian Homeopathic Pharmacopoeia [7]. Medication was diluted in water (1mL/100mL) offered ad libitum, from amber recipient during 16 hours administered 48 hours before infection and 48, 96 and 144 hours after inoculation. Parasitological parameters assessed: total parasitemia (TP), maximum peak of parasites (MPP), pre-patent period (PPP) and area under curve (AUC). Parasitemia was evaluated daily counting from the first day of infection. Clinical parameters assessed: weight, temperature, water and food intake and excreta were measured counting from the 5º day before infection until animals’ death or checking negative parasitemia for 3 consecutive days. Mortality was registered for 75 days after infection. Ethics Committee for Experiments in Animals gave approval UEM 054/11. Statistical comparison of data was performed with Kruskal-Wallis test, with 5% significance.
Results and Discussion: The diluted medications have been significantly reduced the parasitological parameters: MPP (p