Open AccessHigh dilution of Belladonna affect the mycelial growth of Corynespora cassiicola in vitro
Author(s) -
Romulo Vinícios Fagan,
Bruno Reis,
Kátia Regina Freitas Schawan-Estrada,
Carlos Moacir Bonato
Publication year - 2021
Publication title -
international journal of high dilution research
Language(s) - English
Resource type - Journals
ISSN - 1982-6206
DOI - 10.51910/ijhdr.v10i36.510
Subject(s) - corynespora cassiicola , mycelium , serial dilution , horticulture , distilled water , wettable powder , fungicide , potato dextrose agar , biology , petri dish , botany , agar , chemistry , pesticide , agronomy , microbiology and biotechnology , medicine , chromatography , pathology , bacteria , leaf spot , genetics , alternative medicine
The target spot is a disease caused by fungus Corynespora cassiicola (Berk. & Curt.) Wei. This disease has occurred in several states of Brazil. It is a late season disease and causes economic losses in various crops such as soybeans [1]. Currently there is no adequate treatment for the control of C. cassiicola in organic cultivation of soybeans, since the application of fungicides for the control and management of diseases is not allowed by Brazilian legislation [2]. Thus, the purpose of this experiment was to test the effectiveness of high dilutions of Belladonna in vitro on mycelial growth of Corynespora cassiicola.
Materials and Methods: The preliminary tests were conducted at the Laboratory of Plant Pathology, State University of Maringá (UEM). The fungal isolate of C. cassicola was obtained from Embrapa Soja. The fungus was peaked and grown on PDA (potato dextrose agar) maintained at 25°C ± 2 and 12h photoperiod. Belladonna dilutions (6, 12, 18, 24 and 30dH) were obtained according to the Brazilian Homeopathic Pharmacopoeia [3]. PDA culture medium plus Belladonna dilutions (6, 12, 24 and 30dH) beyond the control containing distilled water were placed in petri dishes after filtration through a Millipore membrane (pore diameter of 0.45µm ). After medium solidification, a disc of mycelium (4 mm diameter) of C. cassiicola was peaked towards the center of each plate and sealed with plastic wrap and then incubated at 25°C with 12h photoperiod. The mycelial growth was measured daily for 8 days. The control consisted of distilled water. The data were analyzed by ANOVA and means were compared by Scott-Knott test (P ≤ 0.05).
Results and Discussion: All dilutions of Belladonna (6, 12, 24, 30dH) were effective (p