Open AccessRELATIONSHIP BETWEEN GLUCOSE METABOLISM IN CANCER CELLS WITH DIFFERENT METASTATIC POTENTIAL AND CELL SENSITIVITY TO ANOIKIS
Author(s) -
D L Kolesnik,
O N Pyaskovskaya,
Nataliia Shliakhtova,
G I Solyanik
Publication year - 2021
Publication title -
interconf
Language(s) - English
Resource type - Journals
ISSN - 2709-4685
DOI - 10.51582/interconf.7-8.04.2021.048
Subject(s) - anoikis , glycolysis , cancer cell , cell culture , cancer research , anaerobic glycolysis , extracellular matrix , chemistry , metastasis , cell , metabolism , biology , microbiology and biotechnology , cancer , biochemistry , medicine , genetics
Despite the significant intensification of research on the mechanisms of anoikis, the question of how metastatic cells avoid death upon separation from the extracellular matrix remains open. The aim of the study was to investigate in vitro glucose metabolism in the cells of two variants of Lewis lung carcinoma with different metastatic potential (highly metastatic LLC cells and low metastatic LLC/R9 cells) and to analyze the relationship between metastatic potential, glucose oxidation intensity and cell sensitivity to anoikis. A comparative analysis of the studied indices showed that in conditions of deadhesive growth (which simulated the process of dissemination of metastatically active cells) LLC/R9 cells (as opposed to LLC cells) were characterized by a decreased glycolysis intensity and decreased sensitivity to anoikis. The stability of the glycolysis intensity in LLC cells during the transition to deadhesive growth conditions determined both the resistance (at least in part) of these cells to anoikis and their high metastatic potential.