Antioxidant activity and Gas Chromatography - Mass Spectroscopy analysis of fixed oil from Peganum harmala seeds

The objective of this the study was investigating the chemical constituents and to assess the potential antioxidant of Fixed Oil from Peganum harmala Seeds. Using cold maceration method to extract the Fixed Oil from Peganum harmala Seeds. The chemical constituents of Peganum harmala Oil were identified and quantified by GC-MS, where Antioxidant activities were evaluated using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity. Twenty-three components have been identified. The most abundant fatty acid methyl ester were 9,12, -Octadecadienoic acid (Z,Z) methyl ester with concentration 54.16 % followed by 9- octadecenoic acid (Z) methyl ester with concentration 21.75 %. , Hexadecenoic acid, methyl ester 9.33 %, 11-Octadecenoic acid, methyl ester 2.24 % , Methyl stearate 4.86 %and Stigmasterol 2.76 % .The DPPH assay, showed strong antioxidant potential (94, 0.01 compared with standard 91± 0.01. is a good source of antioxidants due to the presence of phenolic compounds, also is a potential source of natural antibacterial, and justify its uses in folkloric medicine.