Open AccessAnalysis of the Carcinogenic Potential of Helicobacter Pylori Based on Determination of the Degree of Phosphorylation of the Caga Protein Bacteria
Author(s) -
Е. В. Воропаев,
О. В. Осипкина,
О. Ю. Баранов,
A. A. Zyatkov,
Н. А. Бонда,
Э. Н. Платошкин,
А. В. Воропаева,
V. N. Belyakovsky,
С. Л. Ачинович,
А. С. Шафорост,
В. И. Зайцева
Publication year - 2018
Publication title -
problemy zdorovʹâ i èkologii
Language(s) - English
Resource type - Journals
eISSN - 2708-6011
pISSN - 2220-0967
DOI - 10.51523/2708-6011.2018-15-4-17
Subject(s) - caga , helicobacter pylori , polymerase chain reaction , biology , phosphorylation , gene , carcinogen , bacteria , locus (genetics) , microbiology and biotechnology , genetics , virulence
The developed molecular and genetic method for analysis of the carcinogenic potential of Helicobacter pylori based on determining the degree of phosphorylation of the cytotoxin-associated protein of the bacterium (CagA) has been presented. The degree of phosphorylation of CagA protein of Helicobacter pylori is estimated by determining the number and type of so-called EPIYA (Glu-Pro-Ile-Tyr-Ala) motifs at the carboxyl end of the CagA protein region using PCR (polymerase chain reaction) and sequencing of the CagA gene locus. The proposed method can be used to form groups of patients who need additional examination and follow-up observation for the purpose of early prediction of pathological conditions associated with the infectious process caused by Helicobacter pylori strains dominating in the Republic of Belarus. The determination of the number and type of EPIYA motifs can be used as an additional criterion for detection of risk groups for gastrointestinal diseases.