Open AccessTHE MOLECULAR-GENETIC ANALYSIS OF POLYMORPHISM OF IL-4, TNF-α GENES IN PATIENTS WITH LIVER CIRROSIS AND PORTAL HYPERTENSION
Author(s) -
А. Г. Скуратов,
А. Н. Лызиков,
E. V. Voropayev,
О. В. Осипкина
Publication year - 2017
Publication title -
problemy zdorovʹâ i èkologii
Language(s) - English
Resource type - Journals
eISSN - 2708-6011
pISSN - 2220-0967
DOI - 10.51523/2708-6011.2017-14-2-25
Subject(s) - cirrhosis , genotype , gene , snp , tumor necrosis factor alpha , allele , portal hypertension , biology , polymorphism (computer science) , pathological , polymerase chain reaction , gastroenterology , single nucleotide polymorphism , pathology , medicine , immunology , genetics
Objective: to assess the association of polymorphism of the markers of interleukin-4 (IL-4) and tumor necrosis factor (TNF-α) in patients with liver cirrhosis with severe pathological process. Material and methods. The research material was DNA isolated from blood leukocytes of the patients. To identify SNP data, the PCR method was chosen with subsequent restriction analysis and electrophoretic detection by horizontal gel electrophoresis and amplification with visualization of the obtained results. Results. The performed molecular and genetic analysis of IL-4 and TNF-α gene polymorphisms made it possible to assume a relation between the frequency of polymorphic genotypes and alleles of the investigated genes and the severity of liver cirrhosis and portal hypertension, which can be used to diagnose and predict the severe course of the disease.