Open AccessFREE AMINO ACIDS OF BLOOD PLASMA AS AN INTEGRAL PARAMETER OF METABOLIC DISORDERS IN A LONG-TERM INTAKE OF SMALL DOSES OF LEAD ACETATE INTO THE ORGANISM
Author(s) -
V. М. Sheybak,
A. Y. Pavliukovets,
V. Yu. Smirnov
Publication year - 2017
Publication title -
problemy zdorovʹâ i èkologii
Language(s) - English
Resource type - Journals
eISSN - 2708-6011
pISSN - 2220-0967
DOI - 10.51523/2708-6011.2017-14-2-15
Subject(s) - lead acetate , amino acid , isoleucine , arginine , chemistry , methionine , histidine , glycine , blood plasma , threonine , leucine , serine , metabolism , biochemistry , lysine , food science , chromatography , toxicity , organic chemistry , enzyme
Objective: to analyze the pool of free amino acids and their nitrogen-containing metabolites of blood plasma of animals being administered lead acetate for 21 or 28 days. Material and methods. The experiments were carried out on white male rats with the initial weight of 140-160 g. During the experiment the animals received a total amount of 0.67 g of lead acetate (21 days), the second group - 1.12 g (28 days). The determination of free amino acids in blood plasma was performed by the method of reversed-phase HPLC. Results. As a result of the research it has been found out that the most significant contribution to the formation of the amino acid imbalance in blood plasma as a result of the long-term intake of lead acetate according to the value of the Fisher test is caused by changes in the concentrations of α-aminobutyric acid, isoleucine, aspartate, arginine, glycine, serine, histidine, and methionine. Conclusion. The long-term intake of lead acetate with drinking water leads to a statistically significant increase of general content of free amino acids and their nitrogen-containing metabolites, as well as to a decrease of the concentration the essential amino acid threonine in blood plasma.