Open AccessPOST-RESECTION REGENERATION OF THE LIVER IN CASE OF RETRORSIN-INDUCED LESION
Author(s) -
А. Г. Скуратов,
А. Н. Лызиков,
D. R. Petrenyov,
Benjamin Osipov,
С. Л. Ачинович
Publication year - 2016
Publication title -
problemy zdorovʹâ i èkologii
Language(s) - English
Resource type - Journals
eISSN - 2708-6011
pISSN - 2220-0967
DOI - 10.51523/2708-6011.2016-13-1-14
Subject(s) - mesenchymal stem cell , liver regeneration , regeneration (biology) , resection , stem cell , pathology , medicine , adipose tissue , cancer research , transplantation , biology , microbiology and biotechnology , surgery
Objective: to study the regenerative processes of the liver affected by retrorsin after its resection and to assess the reparative role of mesenchymal stem cell transplantation in experiment. Material and methods. Wistar laboratory rats; mesenchymal stem cells from adipose tissue (isolation, cultivation); experimental model of retrorsin-induced hepatitis; liver resection; intraportal introduction of mesenchymal stem cells; light microscopy, morphometry. Results. Using the models of hepatic insufficiency (liver resection, chronic hepatitis) we showed the liver regeneration, whose mechanism involved proliferation of mature hepatocytes (increased mitotic activity, increased cell nuclear device) and biliary ductal epithelial cells. Conclusion. Intraportal administration of autologous MSCs into animals with retrorsin-induced hepatitis contributed to the enhancement of regenerative processes in the liver after its resection in comparison with the control data.