Open AccessГЕОМЕТРИЧЕСКИЕ И ФИЗИКО-МЕХАНИЧЕСКИЕ ПАРАМЕТРЫ ФИБРОБЛАСТОВ И РАКОВЫХ КЛЕТОК ЧЕЛОВЕКА ЛИНИЙ А549 и HEp-2c, ОЦЕНЕННЫЕ С ПОМОЩЬЮ АТОМНО-СИЛОВОЙ МИКРОСКОПИИ
Author(s) -
М. Н. Стародубцева,
И. Е. Стародубцев,
D. R. Petrenyov,
N.I. Yegorenkov
Publication year - 2016
Publication title -
problemy zdorovʹâ i èkologii
Language(s) - English
Resource type - Journals
eISSN - 2708-6011
pISSN - 2220-0967
DOI - 10.51523/2708-6011.2016-13-1-13
Subject(s) - atomic force microscopy , fractal dimension , cytoskeleton , biophysics , materials science , surface finish , chemistry , cell , fractal , biology , composite material , nanotechnology , mathematics , mathematical analysis , biochemistry
Using an atomic force microscopy we studied the physical and mechanical parameters of cell surface layer patches (the parameters of local sliding friction force between atomic force microscope tip and cell surface and the fractal dimension of lateral force maps) and geometrical parameters of whole cells (cell contour, topography, diameter, height and others) for treated with glutaraldehyde (0,5 %) human fibroblasts and cancer epithelial cells lines A549 (lung carcinoma) and HEp-2c (larynx carcinoma). The revealed differences in the studied parameters are indicative of the difference in the cortical cytoskeleton state of the cells.