Open AccessAPPROACHES TO THE ASSESSMENT OF THE BIOLOGICAL AGE BY MEANS OF THE MITOCHONDRIAL DNA ANALYSIS
Author(s) -
E. V. Voropayev,
О. Ю. Баранов,
A. A. Zyatkov,
О. В. Осипкина
Publication year - 2015
Publication title -
problemy zdorovʹâ i èkologii
Language(s) - English
Resource type - Journals
eISSN - 2708-6011
pISSN - 2220-0967
DOI - 10.51523/2708-6011.2015-12-2-13
Subject(s) - mitochondrial dna , polymerase chain reaction , dna , biology , genetics , nuclear dna , microbiology and biotechnology , gene
Objective: to reveal genetic markers acting as predictors of various age-associated changes for their early diagnosis. Material and methods. The study included 35 patients diagnosed with chronic gastritis and examined in the Republican Research Center for Radiation Medicine and Human Ecology (Gomel). Polymerase chain reaction (PCR) and electrophoretic DNA fragment analysis were done. Results. We formed a set of primers for the fragment analysis by 2 loci which made it possible to assess the content of mitochondrial DNA in cells as a marker for senescence. Conclusion. We revealed the dependence of the content of mitochondrial DNA (mtDNA) on the calendar age: the increase of the chronological age leads to the decrease of the mtDNA content.