Open AccessISOLATION OF HEPATOCYTES
Author(s) -
А. Г. Скуратов,
Д. Р. Петренёв
Publication year - 2013
Publication title -
problemy zdorovʹâ i èkologii
Language(s) - English
Resource type - Journals
eISSN - 2708-6011
pISSN - 2220-0967
DOI - 10.51523/2708-6011.2013-10-4-22
Subject(s) - hepatocyte , perfusion , liver perfusion , glycogen , isolation (microbiology) , enzyme , biochemistry , chemistry , microbiology and biotechnology , biology , chromatography , in vitro , medicine
Objective: to study the theoretical basis and give the experimental validation of the efficiency of hepatocyte isolation by the method of enzymatic perfusion of the liver. Material and methods. Wistar rats at a mass of 200 g, isolation of hepatocytes by enzymatic perfusion of the liver, light microscopy, assessment of functional cell capacity to synthesize and accumulate glycogen. Results. The article shows the high efficiency of the method of the 2-step adapted enzymatic perfusion of the liver to isolate hepatocytes. The culture of viable hepatocytes with preserved morphological and functional properties was obtained.