Open AccessCOMET, TUNEL, and TEM analysis of an infertile male with short tail sperm
Author(s) -
Ayşen Durmaz,
Serap Çilaker Mıçılı,
Seda Vatansever,
Cumhur Gündüz,
Hüsnü Alper Bağrıyanık,
Nurten Dikmen,
Ege Nazan Tavmergen Göker,
Erol Tavmergen
Publication year - 2015
Publication title -
journal of the turkish-german gynecological association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.346
H-Index - 16
eISSN - 1309-0399
pISSN - 1309-0380
DOI - 10.5152/jtgga.2014.0017
Subject(s) - sperm , andrology , intracytoplasmic sperm injection , tunel assay , terminal deoxynucleotidyl transferase , human fertilization , male infertility , biology , in vitro fertilisation , comet assay , embryo , dna damage , infertility , anatomy , medicine , pregnancy , genetics , dna , apoptosis
Male infertility is correlated with sperm morphology and sperm DNA damage, which are completely different from that of fertile individuals. An accurate sperm DNA damage analysis and ultrastructural examination of the ejaculate provide important support in the clinical evaluation. It is supposed that in the near future, the fertilization rate, pregnancy rate, and miscarriages could be predicted using the combination of these types of tests in assisted reproductive technologies (ARTs). For this purpose, we report a very rare case of an infertile man having short tail sperm. The infertile man and his wife underwent in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI). During this process, we examined the ultrastructure of the ejaculated sperm with transmission electron microscopy (TEM) and calculated the sperm DNA damage with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and COMET assays. Then, we evaluated the association between sperm DNA damage and embryo quality.