Open AccessRapid Identification of Carbapenemase-producing Klebsiella pneumoniae strains by Matrix-Assisted Laser Desorption/Ionization-Time of Flight using Vitek® Mass Spectrometry System
Author(s) -
Vanessa Gaia Rocco,
Jari Intra,
Cecilia Sarto,
Natalia Tiberti,
Cinzia Savarino,
Maura Brambilla,
Paolo Brambilla
Publication year - 2019
Publication title -
the eurasian journal of medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.337
H-Index - 13
eISSN - 1308-8742
pISSN - 1308-8734
DOI - 10.5152/eurasianjmed.2019.18405
Subject(s) - klebsiella pneumoniae , mass spectrometry , microbiology and biotechnology , plasmid , clinical microbiology , identification (biology) , matrix assisted laser desorption/ionization , computational biology , medicine , biology , chromatography , desorption , escherichia coli , gene , chemistry , genetics , organic chemistry , adsorption , botany
The analysis of the protein pattern of Klebsiella pneumoniae carbapenemase (KPC)-producing strains by Bruker Matrix-Assisted Laser Desorption Ionization (MALDI) Biotyper system has revealed the presence, in the majority of cases, of an 11.109 m/z peak. The peak corresponds to the gene product named p019 of the bla KPC -bearing plasmids and has been suggested as a candidate for a biomarker that is able to distinguish KPC-producers from non-KPC-producers. The aim of this study was to evaluate the rapid detection of the 11.109 m/z peak of KPC-producer strains in the clinical laboratory routine by Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) technique, using the Vitek ® Research-User-Only (RUO) Mass Spectrometry (MS) system without changing the instrument parameters.