Open AccessBIOSYNTHESIS AND CHARACTERIZATION OF ZNO NANOPARTICLES FROM AQUEOUS EXTRACT OF CAMELLIA SINENSIS AND DETERMINE ITS ANTIBACTERIAL ACTIVITY AGAINST MULTIDRUG RESISTANCE BACTERIA
Author(s) -
Ali Hassan Mahmoud,
Zainab Muhammed Nsaif
Publication year - 2020
Publication title -
plant archives/plant archives
Language(s) - English
Resource type - Journals
eISSN - 2581-6063
pISSN - 0972-5210
DOI - 10.51470/plantarchives.2021.v21.s1.422
Subject(s) - camellia sinensis , staphylococcus epidermidis , nuclear chemistry , proteus mirabilis , nanoparticle , antibacterial activity , fourier transform infrared spectroscopy , materials science , aqueous solution , aspergillus flavus , chemistry , microbiology and biotechnology , escherichia coli , bacteria , biology , staphylococcus aureus , nanotechnology , biochemistry , botany , organic chemistry , chemical engineering , genetics , engineering , gene
The current study involved collecting 225 samples of different age groups and from different clinical sources (burns and wounds). Selective and differential media, Microscopic Examination, Biochemical test, IMVIC tests, and Vitek 2 system were used to identify the bacterial species. The results showed that the bacterial isolates were distributed on Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis, Klebsiella pneumoniae, Escherichia coli and Proteus mirabilis. All isolates were multidrug-resistant to 12 antibiotics from different classes and according to the susceptibility test, isolate distinction in 2 groups (75 %) of MDR isolates were resistant to (5-9) antibiotics, while (25 %) were susceptible. Zinc Oxide nanoparticles synthesized was done by a green method with Zinc acetate dehydrate as a precursor and aqueous extract of Camellia sinensisas a reducing agent, color-changing to pale-white was an indication of the formation of ZnONPs. The average size and shape of the nanoparticles were detected by using Atomic Force Microscopy (AFM) which was 40 nm with a spherical shape. Scanning Electron Microscopy (SEM) showed the ZnO NPs have spherical, radial, and cylindrical structures. The wavelength range was measured by Ultraviolet-visible spectroscopy (UV-Vis) for monitoring the formation of the nanoparticles, which showed a sharp peak at 325 nm. The average crystallite size of ZnONPs was estimated using Debye Scherrer’s formula were 20-40nm by using X-ray Diffraction (XRD). Fourier-transform infrared spectroscopy (FT-IR) spectra have been used for ZnONPs to detect the functional groups found in the synthesis process via green tea extract. Keyword: ZnO NPs. Biosynthesis, Camellia sinensis, Green tea, Antibacterial activity