Open AccessBIOCHEMICAL STUDIES IN FOOD SPOILAGE VIA MOLECULAR DETECTION OF SOME ENTEROBACTERIACEAE FOOD BORNE BACTERIA USING MALDI-TOF
Author(s) -
Heba M.Karmy,
Mahfouz A.Abdelaziz,
Doaa Ghaith,
Nashwa M. Helmy,
Kamla M. El–Saied
Publication year - 2020
Publication title -
plant archives/plant archives
Language(s) - English
Resource type - Journals
eISSN - 2581-6063
pISSN - 0972-5210
DOI - 10.51470/plantarchives.2021.v21.s1.407
Subject(s) - salmonella , escherichia coli , enterobacteriaceae , biology , food science , food safety , food microbiology , microbiology and biotechnology , food contaminant , bacteria , escherichia , food spoilage , gene , biochemistry , genetics
Foodborne diseases are caused by consumption of food spoiled by pathogens or their toxins. Sampling beef meat and chicken samples from local markets which is additionally affecting foodborne disease outbreaks, making food safety a universal issue. Biochemical classical microbiological and targeted molecular profiling of Enterobactericea using real time PCR, MALDI TOF as well as sequencing confirmation techniques were performed and compromised for speciation of possible foodborne pathogens in meat and tissue samples in randomly collected 300 samples is traced with their health riskimpact on human. Sum of 68 samples out of 300 were speciated; 28 meat (18 Escherichia coli O157:H7, 10 Salmonella), 18 (10 Escherichia coli O157:H7, 8 Salmonella) and 22 (10 E-coil, 12 Salmonella). Six representative positive samples of both Salmonella and Escherichia coli O157:H7 were selected for further confirmation by sequencing as gold standard technique.