Open AccessISOLATION AND CHARACTERIZATION OF AMMONIA EXCRETING CYANOBACTERIUM CYLINDROSPERMUM SP. NDOP002
Author(s) -
Om Prakash Dwivedi
Publication year - 2021
Publication title -
plant archives/plant archives
Language(s) - English
Resource type - Journals
eISSN - 2581-6063
pISSN - 0972-5210
DOI - 10.51470/plantarchives.2021.v21.no1.187
Subject(s) - ammonia , shoot , chlorophyll , zoology , excretion , horticulture , biology , glutamine , dry weight , paddy field , rice plant , botany , chemistry , agronomy , biochemistry , amino acid
Cylindrospermum sp. NDOP002 was isolated from agricultural fields of Azamgarh, U.P., India. It was characterized by morphological methods. Total chlorophyll a content was 18.5 µg/ mg dry weight. Organism achieved a stationary phase of growth after 15 days of cultivation. Ammonia excretion was monitored from 6 hrs. of culture to 20 days. The ammonia content of culture increased up to 7 days (Maximum amount of 3.23 µg/ ml) and then decreased. Approximately a constant amount of ammonia was maintained from 9th and onwards days of growth. Maximum glutamine synthetase (GS) activity of 8.33 mM ?glutamyl hydroxamate min-1 mg chl-1 was observed on the 5th day of culture and then decreased. Approximately constant GS activity was observed after 9th and onward days of growth. Algalisation experiment showed an increase in growth parameter of rice plant in algalised set. Length of roots was 6.4-6.9 cm in non algalised and 7.2-7.6 cm in algalised rice plants whereas the length of shoots was 5.7-6.1 cm in non- algalised and 9.5-10.1 cm in algalised rice plants. The amount of ammonia in algalised set was 2.1 µg/ ml on the 10th day of the experiment. Experimental findings clearly suggest Cylindrospermum sp. NDOP002 as suitable inocula for algalisation of rice fields of Azamgarh district, U.P., India.