Open AccessINVESTIGATION OF IN VITRO ANTIOXIDANT POTENTIAL OF METHANOLIC EXTRACT OF BARK OF PRUNUS CORNUTA AND ROOT OF RUMEX OBTUSIFOLIUS
Author(s) -
Mahesh C. Purohit,
Navneet Singh,
G. V. S. Kumar,
Rashmi Rawat,
Mahender Singh
Publication year - 2021
Publication title -
plant archives/plant archives
Language(s) - English
Resource type - Journals
eISSN - 2581-6063
pISSN - 0972-5210
DOI - 10.51470/plantarchives.2021.v21.no1.010
Subject(s) - dpph , gallic acid , bark (sound) , chemistry , antioxidant , traditional medicine , prunus , botany , biology , biochemistry , medicine , ecology
In the present research work, in vitro, the antioxidant activity of methanolic extracts of bark of Prunus cornuta and root of Rumex obtusifolius, respectively, was assessed by DPPH free radical scavenging method. Total phenolic content (TPC) of methanolic extracts was also determined by spectrophotometric method using Folin-Ciocalteu reagent and calculated in ?g/mL gallic acid equivalent (GAE). DPPH scavenging activity results show that the root extract of Rumex obtusifolius (IC50 = 17.15) and bark extract of Prunus cornuta (IC50 = 27.32) shows good antioxidant potential. TPC of root extract was found to be 178.56 ?g/g GAE, and bark extract was 137.23 ?g/g GAE. Antioxidant effect (%) determined by using phosphomolybdate assay and superoxide anion radical scavenging percentage further supports the potential antioxidant activity.