Open AccessLimonene and BEZ 235 inhibits growth of COLO-320 and HCT-116 colon cancer cells
Author(s) -
Raja Ratna Reddy Yakkanti,
Pasula Chandra Sekhar,
Kaladhar B. Reddy,
Sonia Ramamoorthy,
Sindhuja Suresh,
T Lakshmi,
Mani Rajesh,
C. Devendranath Reddy
Publication year - 2016
Publication title -
international journal of drug delivery
Language(s) - English
Resource type - Journals
ISSN - 0975-0215
DOI - 10.5138/09750215.1919
Subject(s) - clonogenic assay , cytotoxicity , pharmacology , colorectal cancer , cancer research , cancer cell , cancer , cell cycle , viability assay , cell , medicine , chemistry , in vitro , biochemistry
D-Limonene is a dietary monoterpene with significant anticancer activity against many cancer types in preclinical and clinical studies. The study is designed to investigate synergistic anticancer effects of limonene and BEZ235 combination in COLO-320 and HCT-116 colon cancer cells. Cells were treated with both the drugs alone and in combination and the effects on cell viability; cell migration and clonogenic potential were examined. Results show that both drugs exhibited dose and time dependant cytotoxicity on the cell lines tested. CompuSyn analysis of the drug combination effects revealed the strong synergistic interaction of the combination. Our results also indicate that COLO-320 cells were more sensitive for anticancer effects of the drugs than HCT-116 cells. The presence of Ras and PI3K mutations in HCT-116 cells could possibly be one of the main reasons for the observed outcome as compared to the wild type expressions of them in COLO-320 cells.