Open AccessDevelopment and validation of analytical methods for the simultaneous estimation of Nimorazole and Ofloxacin in tablet dosage form
Author(s) -
A P Ghugare,
Lalchand Devhare,
Bhushan Hatwar
Publication year - 2016
Publication title -
international journal of drug delivery
Language(s) - English
Resource type - Journals
ISSN - 0975-0215
DOI - 10.5138/09750215.1792
Subject(s) - ofloxacin , materials science , absorbance , dosage form , chromatography , distilled water , analytical chemistry (journal) , chemistry , biochemistry , ciprofloxacin , antibiotics
Two simple, rapid, accurate and precise spectrophotometric methods have been developed for simultaneous estimation of Nimorazole and Ofloxacin from tablet dosage form. Method І involves formation of ‘simultaneous equations’ at 304 nm (λ max of Nimorazole) and 287.5 nm (λ max of Ofloxacin); while Method ІІ involves, formation of ‘Absorbance ratio equation’ at 301(isoabsorptive point) and 287.5 nm (λ max of Ofloxacin) using distilled water as a solvent. The linearity was observed in the concentration range of 5 - 25 μg/ml for Nimorazole and 2 - 10 μg/ml for Ofloxacin. The results of analysis have been validated statistically and by recovery studies and were found satisfactory.