Open AccessInvestigation into the effect of the core structure, core-doped, core-doped/shell on the photoluminescence of nanoparticles applied to the detection of E. coli O 157:H7 and methicillin-resistant S. aureus (MRSA)
Author(s) -
Diem Bui Thi,
Liem Nguyen Quang,
Khanh Pham Duy,
Khiem Nguyen Van,
Tang Nguyen Trong,
Bich Luong Thi
Publication year - 2021
Publication title -
xúc tác và hấp phụ
Language(s) - English
Resource type - Journals
ISSN - 0866-7411
DOI - 10.51316/jca.2021.105
Subject(s) - escherichia coli , carbodiimide , chemistry , staphylococcus aureus , photoluminescence , conjugated system , core (optical fiber) , aqueous solution , fluorescence , nuclear chemistry , hydrochloride , nanoparticle , microbiology and biotechnology , nanotechnology , bacteria , materials science , biochemistry , biology , organic chemistry , optoelectronics , physics , genetics , quantum mechanics , composite material , gene , polymer
In this study, Mn-doped ZnSe/ZnS core/shell quantum dots (CSQDs) were synthesized in aqueous solution using 3-Mercaptopropionic Acid as a surface stabilizer and successfully applied in the detection of Escherichia coli O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) for the rst time. The CSQDs were conjugated with anti-E. coli antibody and anti-MRSA antibody via protein A supported by 1-ethyl-3-(-3-dimethylaminopropyl)carbodiimide hydrochloride for uorescent labeling of the intact bacterial cells. The etection was performed for the bacterial strains cultivated in Luria-Bertani liquid medium. The obtained results indicate that E. coli O157:H7 and MRSA can be detected within 30 min at a high sensitivity of 101 CFU/mL. This labeling method based on the highly uorescent CSQDs may have great potential for use in the food industry to check and prevent outbreaks of foodborne illness.