Open AccessPeroxidase-like activity of Fe3O4/carbon core-shell nanostructured : effects of carbon shell thickness for application to glucose biosensor
Author(s) -
Nguyễn Đức Nghĩa,
Huynh Dang Chinh,
Hoang Vinh Tran,
Dao Hong Vu
Publication year - 2021
Publication title -
vietnam journal of catalysis and adsorption
Language(s) - English
Resource type - Journals
ISSN - 0866-7411
DOI - 10.51316/jca.2021.038
Subject(s) - biosensor , hydrogen peroxide , glucose oxidase , horseradish peroxidase , peroxidase , nanocomposite , detection limit , chemistry , selectivity , carbon fibers , nuclear chemistry , catalysis , combinatorial chemistry , nanotechnology , materials science , enzyme , organic chemistry , chromatography , biochemistry , composite material , composite number
In this study, we present a protocol for synthesis of carbon coated Fe3O4 nanoparticles with core-shell structured nanocomposite (FeC) following a two steps approach. The peroxidase-like acitivity of the synthesized FeC nanocomposite has been evaluated towards replacing of the horseradish peroxidase enzyme (HRP) in hydrogen peroxide enzymatic biosensor. In which, FeC has catalyzed for a redox reaction 5,5'-tetramethylbenzidine (TMB) and H2O2 to produce oxidized state of TMB with as a blue color. Results exhibited that FeC has a high catalytic activity accepting for fabrication of a high selectivity hydrogen peroxide (H2O2) colorimetric sensor with low detection of limit (LoD) of 0.02 mM H2O2. Based on this finding, we have used FeC and combined with glucose oxidase (GOx) enzyme to construct a new colorimetric glucose biosensor with high selectivity.
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