Open AccessEFFECT OF THE MAIN CYTOKININS ON ANDROGENESIS OF WHITE CABBAGE (BRASSICA OLERACEA L. var. CAPITATA) ANTHERS CULTIVATED “IN VITRO”
Author(s) -
T. O. Cristea,
Alin Gabriel Iosob,
C. Brezeanu,
Petre Brezeanu,
D. I. Avasiloaiei,
Alexandru Bute
Publication year - 2020
Publication title -
romanian journal of horticulture
Language(s) - English
Resource type - Journals
eISSN - 2734-7664
pISSN - 2734-7656
DOI - 10.51258/rjh.2020.01
Subject(s) - kinetin , zeatin , callus , stamen , brassica oleracea , auxin , biology , cytokinin , botany , brassica , growth regulator , in vitro , explant culture , pollen , genetics , gene
The aim of the present research work was the screening of the effect of the maincytokinin (BAP, kinetin or zeatin) in different concentrations and combinations withthe auxin NAA on androgenesis of white cabbage anthers cultivated in vitro. Theresults obtained are regarded as an intermediary stage for the development of areproducible protocol for in vitro regeneration of plant from anther culture.Thus, for the determination of the influence of plant growth regulators formula overthe callus induction and plant regeneration from anthers cultivated in vitro in thepresent study the authors undergo a screening of the three most frequently utilizedcytokinins (BAP, kinetin and zeatin) in different concentration and combination withthe auxin NAA. The results obtained, indicated that the best morphogenetic reactionis obtained on variant with BAP as the main growth regulator.