Open AccessThe THE FREQUENCY OF MISMATCH REPAIR DEFICIENCY IN COLORECTAL CARCINOMA DETERMINED BY IMMUNOHISTOCHEMISTRY
Author(s) -
Maria Naseem,
Muhammad Asif,
Hafeez Ud Din,
Muhammad Tahir Khadim,
Arshad Khan,
Shahid Jamal,
Iman Shoaib
Publication year - 2021
Publication title -
pakistan armed forces medical journal
Language(s) - English
Resource type - Journals
eISSN - 2411-8842
pISSN - 0030-9648
DOI - 10.51253/pafmj.v71i4.3994
Subject(s) - msh6 , pms2 , medicine , msh2 , mlh1 , immunohistochemistry , lynch syndrome , population , dna mismatch repair , gastroenterology , pathology , colorectal cancer , surgery , cancer , environmental health
Objective: To determine the frequency of mismatch repair deficiency in colorectal carcinoma determined by immunohistochemistry.
Study Design: Cross-sectional study.
Place and Duration of Study: Department of Histopathology, Armed Forces Institute of Pathology, Rawalpindi, from Aug 2018 to Jan 2019.
Methodology: A total of 101 patients of adenocarcinoma of colorectum who underwent surgical resections and their characteristic and clinical data were recorded. Immunohistochemical stains were performed using antibodies MLH1, MSH2, PMS2 and MSH6. Results were interpreted and mismatch repair deficiency status of all patients was determined. Patients with loss of expression for MLH1, MSH2, PMS2 and MSH6 antibodies were observed and noted.
Results: In this study, out of 101 patients with CRC, 71 (70.3%) were male and 30 (29.7%) female. The mean age was (54 years ± 15.9). Amongst the 101 cases loss of immunohistochemical staining for MMR proteins was noted in 19 patients (18.8%). The combined loss of all four antibodies was seen in one case, loss of MLH1 and PMS2 in 7, MSH2 and MSH6 in 5 and MLH1 only in 6 patients. However, no mismatch repair deficiency was detected in remaining 82 cases. According to statistical analysis no significant association between mismatch repair deficiency and variables was found.
Conclusion: The frequency of mismatch repair deficiency in colorectal carcinoma patients was found to be 18.8% in our population.