Open AccessCOMPARATIVE IN- VITRO EVALUATION OF VANCOMYCIN MINIMUM INHIBITORY CONCENTRATIONBY AGAR DILUTION AND E-STRIP IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
Author(s) -
Sheroze Ilyas,
Tehmina Munir,
Rabia Sadaf,
Mehreen Gilani
Publication year - 2021
Publication title -
pakistan armed forces medical journal
Language(s) - English
Resource type - Journals
eISSN - 2411-8842
pISSN - 0030-9648
DOI - 10.51253/pafmj.v71i1.3529
Subject(s) - vancomycin , agar dilution , staphylococcus aureus , agar dilution method , agar , serial dilution , microbiology and biotechnology , minimum inhibitory concentration , medicine , glycopeptide , methicillin resistant staphylococcus aureus , agar plate , biology , antibiotics , bacteria , pathology , alternative medicine , genetics
Objective: To compare the in-vitro efficacy by determining Minimum Inhibitory Concentration of Vancomycinusing the reference Agar Dilution to the E-Strip in Methicillin Resistant Staphylococcus aureus isolates.
Study Design: Validation study.
Place and Duration of Study: The department of Microbiology Army Medical College/National University ofMedical Sciences in collaboration with Pak Emirates Military Hospital Rawalpindi, from Dec 2016 to Dec 2017.
Methodology: Non-duplicate 84 isolates of Methicillin resistant Staphylococcus aureus from various clinical specimens were included in the study. All these isolates were screened for susceptibility to glycopeptide by E-strips method (Bio mérieux) as well as Agar Dilution method, using vancomycin concentrations of 0.25, 0.50, 1.00, 2.00, 4.00 and 8.00µgm/ml respectively in two fold serial dilutions.
Results: There was an overall agreement on 83 samples by both the methods i.e. 83 were Vancomycin SensitiveStaphylococcus aureus by both methods while one isolate with intermediate resistance to Vancomycin was onlydetected by Agar Dilution. The sensitivity of the E–strips compared to Agar Dilution was found to be 100%. Thepositive predictive value was 98.8% with a diagnostic accuracy of 98.8%. Specificity and negative predictive valuecould not be ascertained for E-strips because of the limitation of the method to detect the Vancomycin Intermediate Staphylococcus aureus isolates.
Conclusion: E-strip can be a convenient alternative to the gold standard Agar Dilution but its inability to identifyVISA challenges its reliability in determining the Vancomycin resistance in MRSA isolates.