Open AccessImmobilization of trypsin enzyme on silver nanoparticles
Author(s) -
Sachin Honnenahalli Rajegowda,
Sagar Prabhudev,
D. N. Ganeshprasad,
A.H. Sneharani
Publication year - 2020
Publication title -
biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.106
H-Index - 9
ISSN - 0970-2067
DOI - 10.51248/.v40i2.66
Subject(s) - immobilized enzyme , trypsin , enzyme , chemistry , silver nanoparticle , enzyme assay , chromatography , covalent bond , reusability , nanoparticle , nuclear chemistry , biochemistry , nanotechnology , materials science , organic chemistry , software , computer science , programming language
and Aim:Immobilization of enzyme on silver nanoparticles (AgNPs) is one way to improve their stability and activity and can be reused for large-scaleapplications. The present study was aimed to evaluateand characterizethetrypsin enzyme immobilized on biogenicallysynthesized silver nanoparticles.Materials and
Methods: Immobilization of trypsin enzyme was optimized with time and varying concentration of silver nanoparticles, which were greensynthesized using avocado seedextracts. The residual activity of trypsin enzyme after immobilization was estimated. The reusabilityand temperature stability of the immobilized enzyme were studied.
Results:The immobilized enzyme showed maximum activity of 1006.0 U during the time duration of 18 hr of incubation with AgNPs. Reusability of immobilized enzyme in avocado AgNPs was assessed under optimal conditions, the activity of immobilized enzyme was loss after 5 repeated cycles. The enzyme captured on AgNPswas released in the presence of anionic detergent. Temperaturestability was assessed for immobilized enzyme on AgNPs and the immobilized enzyme was stable up to55ºC and later it loss their activity.
Conclusion: The study reports a feasible method of enzyme immobilization on biogenically synthesized silver nanoparticles. The enzyme immobilized on silver nanoparticles is mainly through non-covalent forces. Further studies are required to improve the reusability of enzyme.
Keywords: Silver nanoparticles;enzyme immobilization;trypsin;temperature stability;reusability;detergent.