Open AccessEvaluation of cytotoxic potential of L-asparaginase from Scopulariopsis brevicaulis on cell lines in vitro
Author(s) -
D’Souza Renita Maria,
Ambily Abraham
Publication year - 2020
Publication title -
biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.106
H-Index - 9
ISSN - 0970-2067
DOI - 10.51248/.v39i2.189
Subject(s) - hela , chemistry , enzyme , cell culture , asparaginase , cytotoxic t cell , ammonium , enzyme assay , chromatography , growth inhibition , extracellular , ammonium sulfate precipitation , microbiology and biotechnology , in vitro , size exclusion chromatography , biochemistry , biology , immunology , organic chemistry , genetics , lymphoblastic leukemia , leukemia
and Aim: This study reports the cytotoxic potential of L-Asparaginase isolated from the fungus Scopulariopsis brevicaulis.
Materials and Methods: Extracellular L- Asparaginase was isolated from Scopulariopsis brevicaulis and purified by ammonium sulfate precipitation, followed by dialysis, ion exchange and gel filtration chromatography. Varying concentrations (31.25, 62.5, 125, 250, 500 µg/ml) of purified L-Asparaginase was tested on MCF7, HeLa, HepG2 and 3T3L1cell lines by MTT assay. Curcumin was maintained as a positive control.
Results: Minimum inhibition of 23.57% was observed at an enzyme concentration of 31.25 µg/ml and maximum inhibition (66.41%) was observed at 500 µg/ml against MCF7 cell line. Minimum inhibition of 2.87% was observed at an enzyme concentration 31.25 µg/ml and maximum inhibition (58.49%) was observed at 500 µg/ml against HeLa cell line. Minimum inhibition of 4.58% was shown at an enzyme concentration of 31.25 µg/ml and maximum inhibition (46.14 %) was observed at 500 µg/ml against HepG2 cell line. Minimum inhibition of 1.4% was shown by enzyme concentration 31.25 µg/ml and maximum inhibition (50.9%) was observed at 500 µg/ml against 3T3L1 cell line.
Conclusion: We report for the first time the cytotoxic potential of L-Asparaginase from Scopulariopsis brevicaulis.