Open AccessCombined Effect of Ethanolic Leaf Extracts of Carica Papaya and Newbouldia Laevis on Hematological Parameters and Sperm Quality of Alloxan-Induced Rats
Author(s) -
Ifegwu Njoku Oji,
Agbai Johnson Ukwa,
Njoku-Oji Njideka Nancy,
Elem Chambelin Jamike,
Aligwekwe Athanasius Ugochukwu,
Ohaeri Esther Adaukwu
Publication year - 2022
Publication title -
international journal of research and scientific innovation
Language(s) - English
Resource type - Journals
eISSN - 2321-2705
pISSN - 2320-5520
DOI - 10.51244/ijrsi.2022.9304
Subject(s) - carica , sperm , sperm motility , epididymis , distilled water , traditional medicine , zoology , biology , medicine , chemistry , botany , chromatography
Objective: This study was carried out to investigate the effect of ethanolic leaf extracts of C. papaya and N. laevis on hematological parameters and sperm quality of alloxan-induced diabetic wistar rats. Methodology: Forty male rats weighing 130-180g were procured, acclimatized for two weeks, after which, were divided into eight groups of five rats each, and were housed in cages. The groups were designated as groups A – H. Group A served as the control group and received distilled water only. Groups B – H were induced with diabetes using alloxan. Group B did not receive any treatment, while the groups C – H received 400mg/kg of C. papaya leaf extract, 600mg/kg of C. papaya leaf extract, 400mg/kg of N. laevis leaf extract, 600mg/kg of N. laevis leaf extract, 200mg/kg of C. papaya + 200mg/kg of N. laevis, and 300mg/kg of C. papaya + 300mg/kg of N. laevis leaf extract respectively for 21 days orally with oral gastric tube. On the 22nd day, the animals were sacrificed via chloroform inhalation and blood samples were collected through ocular puncture for hematological analyses, and epididymis were collected for sperm quality study. All data were tabulated and statistically analyzed using SPSS version 25.0. Result: The levels of WBC, RBC, HGB, PCV, sperm motility and sperm count were significantly (P<0.05) decreased in group B (48.0 ± 2.25) (28.0 ± 2.25) (24.0 ± 1.87) and (50.86 ± 3.18), and D (44.0 ± 3.39) (30.0 ± 2.92) (26.0 ± 2.0) and (42.44 ± 2.17) (for sperm motility and count) when compared to group A (71.0 ± 2.25) (15.0 ± 1.58) (14.0 ± 1.87) and (68.74 ± 2.30), and significantly (P<0.05) increased in groups F (89.0 ± 1.0) (6.0 ± 1.0) (5.0 ± 0.01) and (82.74 ± 3.19), G (91.0 ± 3.30) (5.0 ± 2.81) (4.0 ± 1.8) and (88.78 ± 2.50), and H (88.0 ± 1.60) (7.0 ± 0.80) (5.0 ± 0.01) and (80.92 ± 0.26) ( for sperm motility and count), groups D, F, G and H (for WBC, HGB, PCV) and groups DEFGH (for RBC) when compared to the control group A. However, there was no significant difference on the levels of WBC, RBC, HGB, and PCV, sperm motility and sperm count for groups C and E when compared with the control group A. Conclusion: C. papaya and N. laevis leaf extracts have ameliorating effects on diabetes and increased serum levels of hematological parameters and sperm quality. The ameliorating effects of the combined doses to the diabetic rats were better at lower dosages than when the individual leaf extracts were administered