Open AccessAntibacterial Potential of 2.5% Sodium Hypochlorite in Distinct Irrigation Protocols on Enterococcus faecalis Biofilm
Author(s) -
Denise Ramos Silveira Alves,
Rodrigo Sanches Cunha,
Carlos Eduardo da Silveira Bueno,
Ana Helena Gonçalves de Alencar,
Cyntia Rodrigues de Araújo Estrela,
Tatiane Oliveira Santos,
Carlos Estrela
Publication year - 2015
Publication title -
the journal of contemporary dental practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.3
H-Index - 43
ISSN - 1526-3711
DOI - 10.5005/jp-journals-10024-1687
Subject(s) - enterococcus faecalis , sodium hypochlorite , biofilm , microbiology and biotechnology , enterococcus , hypochlorite , chemistry , biology , bacteria , staphylococcus aureus , antibiotics , organic chemistry , genetics
Objective The aim of this study was to evaluate the effect of irrigation methods on antibacterial potential of 2.5% NaOCl on Enterococcus faecalis biofilm. Materials and methods Enterococcus faecalis biofilms were prepared during 60 days on 48 human root canals and randomized into control and experimental groups using positive and negative pressure irrigation. Bacterial growth was analyzed using turbidity of culture medium followed by UV spectrophotometry, and scanning electron microscopy (SEM) analyses were performed. Mean and standard deviations were used for evaluate the mean optical densities associated to the number of bacteria present culture, and Scheirer-Ray-Hare (an extension of the Kruskal-Wallis test) and Tamhane test to analyze the SEM images in the groups and thirds. Significance was set at 5%. Results Enterococcus faecalis was still present after root canal cleaning regardless of irrigation methods or bacterial identification methods. Conclusion Positive and negative pressure irrigation protocols using 2.5% NaOCl show a similar capacity to reduce E. faecalis in infected root canals. How to cite this article Alves DRS, Cunha RS, da Silveira Bueno CE, de Alencar AHG, de Araújo Estrela CR, dos Santos TO, Estrela C. Antibacterial Potential of 2.5% Sodium Hypochlorite in Distinct Irrigation Protocols on Enterococcus faecalis Biofilm. J Contemp Dent Pract 2015;16(5):340-346.