Open AccessComparison between Tris-buffer and INRA-82 extenders on the quality of chilled rabbit spermatozoa
Author(s) -
Aya M. Fadl,
A. M. Ghallab,
M.M. AbouAhmed
Publication year - 2020
Publication title -
world rabbit science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.396
H-Index - 23
eISSN - 1989-8886
pISSN - 1257-5011
DOI - 10.4995/wrs.2020.12287
Subject(s) - semen , acrosome , andrology , sperm , tris , membrane integrity , extender , chemistry , sperm motility , semen quality , cryopreservation , zoology , biology , biochemistry , medicine , embryo , organic chemistry , membrane , polyurethane , microbiology and biotechnology
The aim of the present study was to compare the effects of tris-buffer and INRA-82 extenders on the quality of cooled rabbit spermatozoa. Pooled semen samples were collected from eight New Zealand White rabbit bucks, divided into three groups and diluted 1:5 with three different semen extenders: INRA-82, tris-citrate glucose (TCG) and tris-citrate trehalose (TCT). Following dilution, the samples were stored at 5°C for up to 48 h. Sperm motility was recorded at 24, 36 and 48 h post-cooling. Furthermore, sperm viability, morphology, membrane integrity, acrosome status and DNA integrity were assessed at 24 h post-cooling. We found that progressive motility percentages were significantly higher ( P <0.05) in samples diluted in INRA-82 (61.00, 53.50, 44.00% at 24, 36 and 48 h post-cooling, respectively) than those diluted in either TCG (39.25, 32.00, 19.75%) or in TCT (47.25, 40.50, 29.00%). We also reported that sperm viability, percentage of normal spermatozoa, percentage of spermatozoa with intact acrosome and DNA integrity after 24 h cooling were significantly higher ( P <0.05) in INRA-82 diluted samples compared to TCG and TCT diluted ones. In summary, our results show that dilution of rabbit semen in INRA-82 improved sperm physiological parameters post-cooling compared to those diluted in TCG or TCT. Our findings also suggest that INRA-82 is a promising diluent that can be used effectively to maintain the viability of chilled rabbit semen.