Open AccessDetection of IgG Antibodies to Glycosylphosphatidylinositol (GPI) as a Biomarker of Immune Status to Plasmodium species
Author(s) -
Asmahani Azira Abdu Sani,
Doko M.H.I.,
M.S. Aliyu
Publication year - 2021
Publication title -
nigerian journal of pure and applied sciences/nigerian journal of pure applied sciences
Language(s) - English
Resource type - Journals
eISSN - 2756-3928
pISSN - 2756-4045
DOI - 10.48198/njpas/21.b12
Subject(s) - asymptomatic , parasitemia , malaria , immunology , antibody , biomarker , giemsa stain , plasmodium falciparum , medicine , immune system , plasmodium (life cycle) , tropical medicine , biology , parasite hosting , pathology , biochemistry , world wide web , computer science
Finding new ways to eliminate malaria is critical and this would greatly be influenced by developing indicators of exposure as well as distribution of effective vaccines against Plasmodium. This study was aimed at detecting Immunoglobulin G(IgG) antibodies, to glycosylphosphatidylinositol (GPI) as a biomarker of immune status to Plasmodium species. In this study, blood samples were gotten from apparently healthy individuals and patients having symptoms of malaria attending Ahmadu Bello University Teaching Hospital, Zaria. Thick and thin blood smears were prepared and stained with Giemsa stain. The smears were observed microscopically. Parasite densities were estimated on positive slides. Samples positive and some negative for Plasmodium were further tested to detect IgG antibodies to GPI among both the Asymptomatic and Symptomatic participants using ELISA. The prevalence of Plasmodium infection among both asymptomatic and symptomatic participants in this study was 18.9% and the prevalence of asymptomatic malaria was 15.6%. There was a significant association between the level of parasitemia and concentration of IgG antibodies to GPI among the asymptomatic participants and a no significant association among symptomatic participants. Type of housing amongst other risk factors was the only factor significantly associated with malaria in this study. This study suggests PGPI as a biomarker of immunity to Plasmodium and may be a vaccine candidate for programs of malaria control.