Open AccessOptimisation of Physical Parameters for Enhanced Antimicrobial Activity of Acalypha Hispida Leaf Extract
Author(s) -
A Banso,
F. Banso,
Sunday Osinkolu Ajeigbe,
A.A. Koleola
Publication year - 2021
Publication title -
nigerian journal of pure and applied sciences/nigerian journal of pure applied sciences
Language(s) - English
Resource type - Journals
eISSN - 2756-3928
pISSN - 2756-4045
DOI - 10.48198/njpas/20.b16
Subject(s) - bacillus subtilis , staphylococcus aureus , antimicrobial , enterococcus faecalis , minimum inhibitory concentration , biology , extraction (chemistry) , traditional medicine , microbiology and biotechnology , escherichia coli , bacteria , antibacterial activity , microorganism , food science , chemistry , chromatography , biochemistry , medicine , genetics , gene
Utilization of available local resources is essential in primary health care. The Genus Acalypha belongs to the family Euphobiaccae. It consists of many species of diverse medicinal uses. The plant is found in differentgeopolitical zones of Nigeria. Extract of Acalypha hispida leaves were examined for their antibacterial effect on Escherichia coli (ATCC 28923), Staphylococcus aureus (ATCC 28923), Enterococcus faecalis (ATCC29212) and Bacillus subtilis (ATCC 6051). Staphylococcus aureus was more susceptible to the effect of A. hispida in susceptibility studies while E. faecalis which has the lowest ranges of suppression appeared to bemore resistant. The minimum inhibitory concentration (MIC) of the extract ranged between 20mg/ml and 35mg/ml. The diameter of zone of inhibition of the extract increased with increase in extraction temperature from 28°C to 80°C when the extract was maintained at these temperatures for 1h. The optimal temperature of extraction for maximum activity of the extract was 80°C while the optimum extraction concentration for maximum activity was 30% (w/v). The extract was thermostable at 45°C temperature. The consequence of theplant extract on the microorganisms suggests that it could be useful in chemotherapy