Open AccessMolecular Characterization of Fungi Associated with Cowdung-impacted Soil
Author(s) -
N.G. Iyanyi,
A. E. Ataga,
C E Akinido,
E Udobong
Publication year - 2020
Publication title -
nigerian journal of pure and applied sciences/nigerian journal of pure applied sciences
Language(s) - English
Resource type - Journals
eISSN - 2756-3928
pISSN - 2756-4045
DOI - 10.48198/njpas/20.b10
Subject(s) - geotrichum , biology , internal transcribed spacer , genbank , polymerase chain reaction , amplicon , dna extraction , cow dung , metagenomics , botany , phylogenetic tree , gene , agronomy , genetics , fertilizer
Dung is the undigested remains of food taken in by herbivores. It is a combination of faeces and urine at a ratioof 3:1. It mostly consists of cellulose, hemicelluloses and lignin. Cow dung harbours several microorganisms,including various species of fungi. The aim of this study was to isolate and characterize the fungal organismsassociated with cow dung-impacted soil using both traditional cultural techniques and molecular method. DNAextraction was carried out using Zymo Quick DNA Fungal/Bacterial Mini prep kit. The Polymerase ChainReaction (PCR) amplification of the Internal Transcribed Spacer (ITS) genes, using the universal primer pair;ITS4 and ITS5, generated amplicon sizes of 372 and 834 base pairs. The amplicons were sequenced using Sangersequencing and the isolates were identified as Lichtheimia ramosa and Galactomyces pseudocandidus.Phylogenetic analysis showed the relationship that exists between the fungal species and other closely-relatedspecies in GenBank such as: Aspergillus amstelodami, Absidia corymbifera, Mycocladus corymbiferus andGeotrichum candidum. This study has provided information on some of the fungal organisms harboured bycowdung-impacted soil which will help predict the possibility for disease transmission to plants or humansthrough cow dung.