Open AccessDNA-Binding and Photocleavage Studies of 9-Phenyl Carbazole
Author(s) -
Hanchate Pallavi,
Oddepally Rajender
Publication year - 2021
Publication title -
international journal of advanced research in science, communication and technology
Language(s) - English
Resource type - Journals
ISSN - 2581-9429
DOI - 10.48175/ijarsct-2370
Subject(s) - carbazole , intercalation (chemistry) , dna , circular dichroism , chemistry , binding constant , base pair , crystallography , fluorescence , cleavage (geology) , stereochemistry , biophysics , binding site , photochemistry , materials science , biochemistry , organic chemistry , biology , physics , quantum mechanics , fracture (geology) , composite material
The DNA-binding mode of 9-Phenyl carbazole with CT-DNA was investigated by absorption spectroscopy, EB-DNA displacement, circular dichroism, thermal denaturation and viscosity measurements. Results indicated that these compounds intercalate into the base pairs of CT-DNA. The effect of ionic strength on the fluorescence property of the system indicated the presence of electrostatic interaction via phosphate backbone of DNA helix. The intrinsic binding constant values suggested that compound has DNA binding propensity. This compound promote the cleavage of plasmid pBR322. These results may be useful for the design of 9-Phenyl carbazole with desired binding characteristics and useful to better understand the DNA binding mode of heterocyclic compound.