Open AccessAnti-Inflammatory Activity Of Polyphenols From Olive Oil Mill Wastewaters
Author(s) -
Zakia Gueboudji
Publication year - 2022
Publication title -
jordanian journal of engineering and chemical industries
Language(s) - English
Resource type - Journals
eISSN - 2617-720X
pISSN - 2616-9584
DOI - 10.48103/jjeci542022
Subject(s) - polyphenol , chemistry , diclofenac sodium , maceration (sewage) , food science , antioxidant , extraction (chemistry) , phenols , ic50 , olive oil , chromatography , traditional medicine , in vitro , biochemistry , medicine , materials science , composite material
Olive oil mill wastewater (OMW) is a by-product of the olive oil industry. It is very polluting and causes serious environmental problems, but is very loaded with polyphenols, which have antioxidant and anti-inflammatory power. This study aimed to evaluate in vitro the anti-inflammatory potential of the phenolic extract of OMW from two varieties, Chemlal and Zlitni, extracted from a modern olive mill located in Khenchela, eastern Algeria. The maceration method did the extraction of the polyphenols. The dosage of total polyphenols and flavonoids content was determined by Folin-Ciocalteuand aluminum trichloride methods successively. Two tests made the anti-inflammatory activity, inhibition of protein denaturation (IPD) and membrane-stabilizing potential (MSP), comparing with sodium diclofenac. The results obtained showed that the phenolic extract of OMW of Chemlal was found to exhibit the highest inhibitory effect anti-inflammatory activity (IPD: IC50 = 91.27 ± 2.73 µg/mL, and MSP: IC50=98.41 ± 1.22 µg/mL) more than the phenolic extract of Zlitni and the standard used (sodium iclofenac). The in vitro assays carried out show that the phenolic extract of OMW has animportant source of natural anti-inflammatory agents, which can be used in the pharmaceutical industry and on the other hand reducing its dangerous impact on the environment.