Open AccessAssembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men
Author(s) -
Tamara Janković,
Jelena Lukovic,
Irena Miler,
Nevena Mitić,
Ljiljana Hajduković,
Miroslava Janković
Publication year - 2021
Publication title -
upsala journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.808
H-Index - 41
eISSN - 2000-1967
pISSN - 0300-9734
DOI - 10.48101/ujms.v126.7673
Subject(s) - medicine , glycan , galectin , microbiology and biotechnology , andrology , glycoprotein , immunology , biology
Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes. In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.