Open AccessCryopreservation of Dendrobium cruentum Rchb. f. Seeds by D Cryo-plate and V Cryo-plate Techniques
Author(s) -
Sasikarn Prasongsom,
K. Thammasiri,
Jarunya Narangajavana,
Siripong Thitamadee,
Ngarmnij Chuenboonngarm,
Nathinee Panvisavas
Publication year - 2019
Publication title -
walailak journal of science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.146
H-Index - 15
eISSN - 2228-835X
pISSN - 1686-3933
DOI - 10.48048/wjst.2020.6159
Subject(s) - cryopreservation , germination , dehydration , horticulture , botany , biology , microbiology and biotechnology , embryo , biochemistry
Dendrobium cruentum Rchb. f. is a native Thai orchid species that has faced extinction because of its attractive characteristics. Consequently, conservation of this species is urgently needed. In this study, cryopreservation technique was applied to D. cruentum Rchb. f. seeds for long-term conservation. A successful protocol for D. cruentum Rchb. f. seed cryopreservation was developed by using D cryo-plate and V cryo-plate techniques. Seed viability was tested by TCC solution and 93.8 % of dyed seeds were shown. For cryo-plate technique, seeds were encapsulated over the cryo-plate by using 2 % (w/v) sodium alginate and polymerized with 100 mM CaCl2. Encapsulated seeds were desiccated by using a laminar airflow and PVS2 solution treatment with the same exposure time (0, 30, 60, 90, and 120 min). After cryopreservation, encapsulated seeds were cultured on modified VW agar medium. From the results, the maximum germination and regrowth percentage were observed; D cryo-plate technique with 60 min of dehydration time gave the highest germination (68.9 %) and regrowth (57.8 %). Thus, the excess of dehydration may cause the reduction of germination and plant regeneration. In conclusion, D cryo-plate technique proved to be appropriate for D. cruentum Rchb. f. seed cryopreservation.