Open AccessAnti-Methicillin Resistant Staphylococcus aureus (MRSA) Activity of Pseudoalteromonas flavipulchra Isolated from Marine Waters of Batangas, Philippines
Author(s) -
Arizaldo E. Castro,
Cristina C. Salibay
Publication year - 2020
Publication title -
walailak journal of science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.146
H-Index - 15
eISSN - 2228-835X
pISSN - 1686-3933
DOI - 10.48048/wjst.2020.4789
Subject(s) - pseudoalteromonas , staphylococcus aureus , broth microdilution , microbiology and biotechnology , biology , methicillin resistant staphylococcus aureus , minimum inhibitory concentration , isolation (microbiology) , antibiotics , bacteria , 16s ribosomal rna , genetics
The ocean boasts untapped novel producers of antibiotic substances in the form of unicellular organisms. One of the newest bioproducers of pharmacologically-significant substances studied for its potential against clinically-significant pathogens is the genus Pseudoalteromonas, a gammaproteobacterial group. This study is a preliminary report detailing the isolation of a Pseudoalteromonas flavipulchra species from Philippine marine waters. The isolate coded as PAM-003 was identified as 100 % similar to P. flavipulchra strain NCIMB2033 through 16s rRNA gene amplification and sequencing. PAM-003 was allowed to produce bioactives for 12 days. Afterwards, non-polar products were isolated from the base medium through membrane filtration, organic solvent extraction and rotary evaporation. The crude solution of bioactives injected in sterile discs was used for disc-diffusion assay against Methicillin Resistant Staphylococcus aureus (MRSA). Results indicate that PAM-003 demonstrated visually-appreciable zones of inhibition with a mean value of 8 mm. To further describe the antibacterial activity of the isolate, minimum inhibitory concentration (MIC) of the bacterial extract was determined through broth microdilution technique. Results indicate that PAM-003 demonstrated a MIC of 1000 µg/mL against MRSA. Additional investigation on the bioactivity of Philippine isolates from the genus Pseudoalteromonas isolated from highly diverse regions of the country is a considerable initiative for increasing the pipeline of new molecular entities for drug discovery.